Flavones & C3G


Methoxyisoflavone (5-methyl-7-methoxyisoflavone) and ipriflavone (7-isopropoxyisoflavone) are claimed to be powerful anabolic isoflavones similar to those found in soy. They are not hormones but according to Chinoin Research, they display anti-catabolic and enhanced protein synthesis qualities; like androgens. Methoxyisoflavone was designed to combat “muscle-wasting” conditions by reducing cortisol, increasing protein synthesis and blocking oestrogen conversion.

Ipriflavone is claimed to display anabolic steroid potency but without the side effects; increased protein synthesis, nitrogen retention and anti-catabolism. These two isoflavones are synergistic (work with each other) and when combined give enhanced effects.

We at Physique Bodyshop enjoy researching into supplements to validate (or challenge) such claims, especially of this nature! We went to the patent application to see what research was done to make such claims: The patent applications for ipriflavone and Methoxyisoflavone make them look like promising compounds for increasing muscle mass. The ipriflavone patent describes a number of tests. It indicates that ipriflavone at 30 mg/kg was anabolic to muscle tissue without androgenic effects using rats, although the degree of the anabolic effect was not given. It also indicates a significant reduction in nitrogen excretion, increased methionine incorporation into muscle tissue, resistance to the catabolic effect of cortisol (with a greater effect than anabolic steroids), and increased weight gain in animals after ipriflavone administration. Ipriflavone caused weight gain in calves, cattle, hogs, poultry, rabbits, and guinea pigs, all without increased food intake.

The patent for Methoxyflavone examines its effects and the effects of some other derivatives of ipriflavone and compares them to those of ipriflavone. Of these compounds, methoxy was the most potent. All of the compounds increased the retention of calcium, phosphorus, potassium, and nitrogen. They also report that these compounds lacked negative androgenic, oestrogenic, CNS, and hepatotoxic (liver) effects and reduced cholesterol. Finally, when given to chickens, these substances caused significant weight gain over control and ipriflavone.

There are a number of reasons to treat this information with caution. First, the information provided in patent applications is often unreliable and bias. Second, a mechanism of action for how these anabolic effects are achieved is not described. Third, although it would seem that an anabolic effect on muscle tissue is demonstrated, the degree of this effect is not given. It could be that there is only a small effect, and the majority of the weight gain caused by these isoflavones is from bone or another tissue. Also, ipriflavone and its metabolites appear to accumulate primarily in the gastrointestinal tract, liver, kidneys, bones, and adrenal glands, although this does not rule out the possibility of an indirect effect.

Dosage and side effects

Ipriflavone is generally well tolerated and free of side effects.

Since there is little research on methoxyisoflavone, the safety and side effect profile, other than what was previously mentioned from the patent application, is largely unknown.

For ipriflavone, the dose used in most clinical trials has been 600 mg daily (200 mg three times daily), and this dosage should be lowered in those with kidney disorders. It is also normally taken with a calcium supplement. Many users of methoxy and ipriflavone for body composition/aesthetic purposes feel they get the best results with 1-2 grams daily.

Indigo-3G, Cyanidin-3-Glucoside

So, “Scientists have discovered a compound that precisely controls nutrients so what you eat literally turns to muscle instead of fat.”…. REALLY? Utter market hype! And expensive!

Indigo-3G, is highly purified Cyanidin-3-Glucoside (C3G). It is an anthocyanin which is a class of flavonoids and is a pigment producing chemical in several flowers and fruits like blueberries and blood oranges. Like many fruit based chemicals, it is an extremely strong antioxidant.

The article over at T-Nation claims that ingesting C3G will turn you into a genetic freak. You know those people that can eat whatever they want and stay lean? That could be you. In fact, they recommend eating more than you usually do, and you will still get lean! This is obviously marketing hype, and that’s not to blast Biotest. The supplement game IN GENERAL is based around bloated claims like this. Every supplement out there would be disqualified if it was your only point of judgment. Even bodybuilders on anabolic steroids have to specifically diet and train to lose weight. Manage your expectations.

So does this stuff work…. The answer is that no one really knows outside of the original Biotest guinea pigs who have a vested interest in the company. There are currently no in vivo, human studies on C3G, but rat studies and in vitro human cell studies can show whether there is at least a chance that C3G could work. The article on T-Nation lists several of these.